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|
Table 1. Total protein concentration on each resulting fraction from the purification procedure. |
|
Samples |
[mg/ml] |
V (ml) |
Total protein [mg] |
% From initial |
|
Purified gills (40 g) |
||||
|
Acid Extract |
3.36 |
400.0 |
1,346.40 |
----- |
|
Eluted SE fraction |
3.78 |
12.0 |
55.00 |
4.09 |
|
Eluted Sep-pak C-18 ® |
||||
|
5% ACN |
0.88 |
0.4 |
0.35 |
0.02 |
|
20% CAN* |
40.00 |
0.4 |
16.00 |
1.18 |
|
40% ACN |
89.00 |
0.4 |
35.60 |
2.64 |
|
60% ACN |
3.56 |
0.4 |
1.42 |
0.10 |
|
80% ACN |
0.625 |
0.4 |
0.25 |
0.18 |
| *The 20 % ACN fraction was further processed through RP-HPLC. |
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|