doi: 10.2225/vol12-issue2-fulltext-11
Electronic Journal of Biotechnology, Vol 12, No 2 (2009)
A rapid and cheap protocol for preparation of PCR templates in peanut
Abstract
This paper describes a simple, low cost and reliable DNA template preparation protocol for polymerase chain reaction (PCR) using immature leaves from peanut seeds or leaves from field-grown plants. The technique may find wide utility in studies involving PCR-based molecular markers, rapid screening for transformants and gene cloning.